產品名稱:Anti-HMGCLL1抗體,HMGCLL1蛋白抗體
產品編號:BYK-18053R
英文名稱:Anti-HMGCLL1
中文名稱:HMGCLL1蛋白抗體
英文別名:3 hydroxymethyl 3 methylglutaryl CoA lyase like protein 1;
3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase like 1;
Probable 3 hydroxymethyl 3 methylglutaryl CoA lyase 2.
英文介紹:The 3-hydroxymethyl-3-methylglutaryl-CoA lyase-like protein 1 (HMGCLL1) is a 370 amino acid protein that belongs to the HMG-CoA lyase family and is involved in the catabolism of branched amino acids, such as leucine and isoleucine. The gene encoding HMGCLL1 maps to human chromosome 6, which contains around 1,200 genes within 170 million base pairs of sequence. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer suggesting the presence of a cancer susceptibility locus. Porphyria cutanea tarda is associated with chromosome 6 through the HFE gene which, when mutated, predisposes an individual to developing this porphyria. Notably, the PARK2 gene, which is associated with Parkinson's disease, and the genes encoding the major histocompatiblity complex proteins, which are key molecular components of the immune system and determine predisposition to rheumatic diseases, are also located on chromosome 6.
產品規格:0.1ml/0.2ml
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯免疫(ELISA),IF、IP、流式細胞術等實驗(可來電索取說明)
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-HMGCLL1抗體,HMGCLL1蛋白抗體免疫組化操作流程
1 脫蠟和水化:脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復:用于福爾馬林固定的石蠟包埋組織芯片:
A 抗原熱修復
a 高壓熱修復 在沸水中加入EDTA(ph8.0)或0.01m*緩沖溶液(ph6.0).蓋上不銹鋼鍋蓋,但不能鎖定。將玻片置于金屬染色加上,緩慢加壓,是玻片在緩沖液中浸泡五分鐘,然后將蓋子鎖定,小閥門將會升起來。10分鐘后除去熱源,置入涼水中,當小閥門沉下去后打開蓋子。此方法適用于較難檢測或核抗原的抗原修復。
b 沸熱修復 電爐或水浴鍋加熱0.01*緩沖液(ph6.0)至95℃左右,放入組織芯片加熱10-15分鐘。
C 微波爐加熱 在微波爐里加熱0.01*緩沖液(ph6.0)至沸騰后將組織芯片放入,斷電,間隔5-10分鐘,反復1-2次。適用的抗原:
Bcl-2.Bax.AR.PR.C-fos.x-jum.z-kit.c-myc.E-cadherin.
ChromograninA.Cyclin.ER.Heatshock.Protein.HPV.Ki-67.MDMZ.P53.P34.P15.P-glycoprotein.PKC.PCNA.ras.Rb.等
B 酶消化方法
常用0.1%*和0.4%*液。*使用前預熱37℃,消化時間約為5-30分鐘。適用于被固定遮蔽的抗原:包括 Collagen.GFAP.
Complement.Cytokeratin.C-erB-2.LCA.LN.等
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