產品名稱：Anti-ornithine aminotransferase抗體,*氨基轉移酶抗體

產品編號：BYK-18036R
英文名稱：Anti-ornithine aminotransferase
中文名稱：*氨基轉移酶抗體
英文別名：GACR;

Gyrate atrophy;

HOGA; OAT_HUMAN; Ornithine aminotransferase, hepatic form; Ornithine aminotransferase, renal form;

OAT;

OATASE;

OKT;

Ornithine aminotransferase (gyrate atrophy);

Ornithine aminotransferase mitochondrial;

ornithine aminotransferase precursor;

Ornithine aminotransferase, mitochondrial precursor;

Ornithine delta aminotransferase;

Ornithine oxo acid aminotransferase.
英文介紹：OAT (ornithine aminotransferase (mitochondrial), ornithine-oxo-acid aminotransferase) is a 439 amino acid protein encoded by the human gene OAT. OAT belongs to the class III pyridoxal-phosphate-dependent aminotransferase family and is usually found as a homotetramer in the mitochondrion matrix. OAT catalyzes the major catalytic reaction for ornithine. Ornithinemia, presumably due to deficiency of ornithine ketoacid aminotransferase (OAT) has been found in patients with gyrate atrophy of the choroid and retina. The clinical history of gyrate atrophy is usually night blindness that begins in late childhood, accompanied by sharply demarcated circular areas of chorioretinal atrophy. During the second and third decades the areas of atrophy enlarge. The hepatic cleavage product, hepatic OAT, is formed by cleaving a 25 amino acid transit peptide from the N-terminus of the OAT precursor. The renal form is produced by cleaving a 35 amino acid transit peptide from the N-terminus。

產品規格：0.1ml/0.2ml

注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯免疫(ELISA)，IF、IP、流式細胞術等實驗（可來電索取說明）
此產品本公司有標記的一抗出售，相關標記有：Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記

保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-ornithine aminotransferase抗體,*氨基轉移酶抗體免疫組化操作流程
1 脫蠟和水化：脫蠟前應將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘。 a 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b 無水乙醇中浸泡五分鐘
c 95%乙醇中浸泡五分鐘
d 75%乙醇中浸泡五分鐘
2 抗原修復：用于福爾馬林固定的石蠟包埋組織芯片：
A 抗原熱修復
a 高壓熱修復 在沸水中加入EDTA（ph8.0）或0.01m*緩沖溶液（ph6.0）.蓋上不銹鋼鍋蓋，但不能鎖定。將玻片置于金屬染色加上，緩慢加壓，是玻片在緩沖液中浸泡五分鐘，然后將蓋子鎖定，小閥門將會升起來。10分鐘后除去熱源，置入涼水中，當小閥門沉下去后打開蓋子。此方法適用于較難檢測或核抗原的抗原修復。
b 沸熱修復 電爐或水浴鍋加熱0.01*緩沖液（ph6.0）至95℃左右，放入組織芯片加熱10-15分鐘。
C 微波爐加熱 在微波爐里加熱0.01*緩沖液（ph6.0）至沸騰后將組織芯片放入，斷電，間隔5-10分鐘，反復1-2次。適用的抗原：
Bcl-2.Bax.AR.PR.C-fos.x-jum.z-kit.c-myc.E-cadherin.
ChromograninA.Cyclin.ER.Heatshock.Protein.HPV.Ki-67.MDMZ.P53.P34.P15.P-glycoprotein.PKC.PCNA.ras.Rb.等
B 酶消化方法 
常用0.1%*和0.4%*液。*使用前預熱37℃，消化時間約為5-30分鐘。適用于被固定遮蔽的抗原：包括 Collagen.GFAP.
Complement.Cytokeratin.C-erB-2.LCA.LN.等

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