［產品名稱］大鼠胰島素（Insulin）ELISA檢測試劑盒
［英文名稱］Rat Insulin ELISA KIT
［貨號］ZK-R3059
［試劑盒］保存條件、保質期2-8℃低溫保存下，6個月的保質期。
［運輸方式］當地客戶可以由專門配送人員免費送貨上門貨快遞，外地客戶當天下單當天免費快遞送貨上門。
［技術服務］凡購買子科生物子科生物任何一款ELISA酶聯免疫分析檢測試劑盒，都可以享受免費代測服務，外地客戶可以由技術老師的指導下正確放置好樣本的保存方式后快遞郵寄到我公司技術部，本地客戶可以享受免費上門取樣服務！
［產品價格］詳細價格可以咨詢在線客服2811391951，2016年年中*！或  進行詢價，可以享受7折優惠！

大鼠胰島素（Insulin）ELISA檢測試劑盒購買子科生物ELISA系列產品優勢：
1）每個產品出庫前都會進行質檢，質檢合格后才可出庫，以確保每個產品質量合格，以避免出現售后服務。
2）產品經過技術人員多年的研發經驗調試和修正，讓產品實驗效果穩定，靈敏度高，重復性高，吸附性能好，空白值低，孔底透明度高，且*。
3）產品種類齊全，包括人、大鼠、小鼠、豚鼠、兔子、植物、山羊、綿羊、牛、猴、豬、雞鴨、魚、昆蟲等各種動植物等種屬ELISA試劑盒，能夠滿足廣大科研工作者的需要，讓您可以在本公司體會一站式消費體驗。
4）快速，檢測時間短（2-3小時即可完成），幫您節省寶貴的實驗時間。
5）免費提供ELISA試劑盒免費代測服務，和數據分析工作。需要代測的客戶只需要準備好您的樣本，郵寄到我們公司實驗室即可，本市可以安排工作人員上門取樣本，我們承諾在5個工作日內給您zui終檢測結果。
6）適用范圍廣：可以檢測血清、血漿、各組織樣本、細胞培養上清液、尿液、腦脊液、糞便等等多種標本類型；
7）快速：我們深圳子科生物有一個龐大的現貨倉庫，客戶凡當天下單都可以當天發貨，保證發貨及時。
8）我公司擁有專業的技術老師，能確保良好快速的處理售前售中及售后服務。

Assay procedure
1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.
3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.
4.  Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 
5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not 
appear uniform, gently tap the plate to ensure thorough mixing.
8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.

Calculation of results
1.This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 
2.First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 
3.To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 
4.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
5.The sensitivity by this assay is 10.0 pg/ml
6.Standard curve
Storage：  2-8℃.
validity： six months.

其他相關產品如下：ZIKER-0595R    Anti-DMBT1 (Deleted in malignant brain tumor 1)  抑癌基因抗體
ZIKER-0678R    Anti-Dnmt1(DNA methyltransferase 1)  DNA甲基轉移酶1抗體
ZIKER-0497R    Anti-Dnmt3a(cytosine-5)-methyltransferase 3A)  DNA甲基轉移酶-3α抗體
ZIKER-0301R    Anti-Dnmt3β (DNA (cytosine-5)-methyltransferase 3 β)  DNA甲基轉移酶-3β抗體
ZIKER-0597R    Anti-DPPA2 (developmental pluripotency associated gene2)  多能發育相關基因2抗體
ZIKER-0421R    Anti-DR3/TNF-αR/TRAMP (death receptor 3)  TNF-α膜受體抗體/DR3 死亡受體3抗體
ZIKER-0591R    Anti-DR4/APO2/TRAILR1 (Death receptor 4)  死亡受體4抗體
ZIKER-0775R    Anti-DTNBP1 (Dysbindin-1)  精神分裂癥易感基因抗體
ZIKER-0598R    Anti-DVL1 (dishevelled 1)  蓬亂蛋白1抗體
ZIKERh-0574M    Anti-Dynamin 2（Mouse Anti-Human Dynamin-2 Monoclonal Antibody）  鼠抗人鳥苷三磷酸酶－發動蛋白2單克隆抗體
ZIKER-0574R    Anti-Dynamin 2（Anti-Human Dynamin-2 ）  兔抗人鳥苷三磷酸酶－發動蛋白2抗體
ZIKER-0831R    Anti-Dysbindin/rhogap92b  精神分裂癥易感基因抗體
ZIKER-0599R    Anti-E2F1  轉錄因子E2F-1抗體
ZIKER-1001R    Anti-E2F2  轉錄因子E2F-2抗體
ZIKER-1003R    Anti-EAAT1/Glast(Excitatory amino acid transporters 1)  膠質細胞谷氨酸運載蛋白1 抗體
ZIKER-0820R    Anti-EBNA-3 (EpsteinZIKERrr nuclear antigens 3)  EB病毒編碼核蛋白-3抗體
ZIKER-1190R    Anti-ECE1（Endothelin Converting Enzyme 1）  內皮素轉化酶1ECE1抗體
ZIKER-0500R    Anti-ECG2 (sophagus cancer-related gene-2)  食道癌相關基因抗體
ZIKER-0776R    Anti-ECM1 (extracellular matrix protein 1)  細胞外基質蛋白1抗體
ZIKER-1149R    Anti-ED-1 (Ectodermal Dysplasia 1)  外胚層發育不良抗體
ZIKER-0165R    Anti-EGF-R （Epidemal growth factor receptor）  表皮生長因子受體抗體
ZIKER-0405R    Anti-EGFR-ⅤⅢ  表皮生長因子受體-8抗體
ZIKER-1076R    Anti-Egr-1 （Early growth response 1）  早期反應基因-1/應急反應生長基因1抗體
ZIKER-0558R    Anti-Emp1 (epithelial membrane protein 1)  表皮膜蛋白1抗體
ZIKER-1331R    anti-EMAb（Endometrium）  抗子宮內膜抗體
ZIKER-0547R    Anti-Endostatin  內皮抑素/內皮他丁抗體
ZIKER-1249R    Anti-EpCAM（Epithelial cell adhesion molecule）  上皮細胞粘附分子/表皮細胞粘附分子抗體