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Human interleukin 18（IL-18）

時間：2011/1/5閱讀：405
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Principle of the assay
The kit assay Human IL-18level in the sample，use Purified Human IL-18antibody to coat microtiter plate wells, make solid-phase antibody, then addIL-18to wells,CombinedIL-18 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Compley,Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed,reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration ofHuman IL-18in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit

1
wash solution
20ml×1bottle
7
Stopp Solution
6ml×1 bottle
2
HRP-Conjugate reagent
6ml×1 bottle
8
Standard（320ng/L）
0.5ml×1 bottle
3
Microelisa stripplate
12well×8strips
9
Standard diluent
1.5ml×1bottle
4
Sample diluent
6ml×1 bottle
10
Instruction
1
5
Chromogen Solution A
6ml×1 bottle
11
Closure plate membrane
2
6
Chromogen Solution B
6ml×1 bottle
12
Sealed bags
1

Specimen requirements

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