豬胰島素(INS)ELISA試劑盒
Porcine Insulin(INS)ELISA Kit
實驗原理：
本試劑盒僅供研究使用，用于測定豬血清，血漿及相關液體樣本中胰島素(INS)的含量。應用雙抗體夾心法測定標本中豬胰島素(INS)水平。用純化的豬胰島素(INS)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入胰島素(INS)，再與HRP標記的胰島素(INS)抗體結(jié)合，形成抗體-抗原-酶標抗體復合物，經(jīng)過*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的胰島素(INS)呈正相關。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中豬胰島素(INS)濃度。
Principle of the assay
The kit assay Porcine INS level in the sample，use Purified Porcine INS to coat microtiter plate wells, make solid-phase antibody, then add INS to wells, Combined INS which With HRP labeled ， become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of INS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
試劑盒供應商：上海華壹生物科技有限公司
