GATA結(jié)合蛋白4抗體 Anti-GATA4相關(guān)標(biāo)記     Alexa Fluor 350   Alexa Fluor 488   Alexa Fluor 555   Alexa Fluor 647   AP   APC   Biotin   Cy3   Cy5  

Cy5.5   Cy7   FITC   Gold   HRP   PE   PE-Cy3   PE-CY5   PE-CY5.5   PE-CY7   RBITC  
英文名稱     Anti-GATA4
中文名稱     GATA結(jié)合蛋白4抗體
別    名     GATA binding factor 4; GATA 4; GATA4; GATA-4; Transcription factor GATA-4; GATA-binding factor 4; Gata4; GATA4_HUMAN; GATA-binding factor 4. 
  GATA結(jié)合蛋白4抗體 Anti-GATA4抗體來源     Rabbit
克隆類型     polyclonal
交叉反應(yīng)     Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, 
產(chǎn)品類型     一抗  
研究領(lǐng)域     心血管 免疫學(xué) 發(fā)育生物學(xué) 干細(xì)胞 轉(zhuǎn)錄調(diào)節(jié)因子 鋅指蛋白 表觀遺傳學(xué)
蛋白分子量     predicted molecular weight: 49kDa
性    狀     Lyophilized or Liquid
免 疫 原     KLH conjugated synthetic peptide derived from human GATA-4 (301-332aa)
亞    型     IgG
純化方法     affinity purified by Protein A
儲 存 液     0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
產(chǎn)品應(yīng)用      WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500 
（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.
保存條件     Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note     This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 產(chǎn)品介紹    GATA4 is a 46 kDa member of the GATA family of zinc finger containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs.
Function : Transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3'. Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement.
Subunit : Interacts with ZNF260 (By similarity). Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with NFATC4 and LMCD1 (By similarity). Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes.
Subcellular Location : Nucleus.
Post-translational modifications : Methylation at Lys-300 attenuates transcriptional activity.
DISEASE : Defects in GATA4 are the cause of atrial septal defect type 2 (ASD2) [MIM:607941]. ASD2 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. ASD2 patients show other heart abnormalities including ventricular and atrioventricular septal defects, pulmonary valve thickening or insufficiency of the cardiac valves. ASD2 is not associated with defects in the cardiac conduction system or non-cardiac abnormalities.
Similarity : Contains 2 GATA-type zinc fingers.
Database links : UniProtKB/Swiss-Prot: P43694.2
GATA-4屬于轉(zhuǎn)錄調(diào)節(jié)性因子，表達(dá)在心組織、胃腸道組織中，并調(diào)節(jié)該類T細(xì)胞的發(fā)育和功能。