人皮膚淋巴細胞相關抗原(CLA)ELISA試劑盒運輸方法：泡沫箱配生物冰袋運輸
價格及詳細資料：電議,或咨詢在線客服,或者以郵件形式發到我司qysw@qiyibio.com .齊一生物科技（上海）有限公司提供ELISA試劑盒受到了廣大科研單位*肯定和認同。*保證，價格公道，傾力為國內外科研院校實驗室提供產品。若有需要，我司將竭誠為您服務！
FOR RESEARCH USE ONLY
Drug Names
Generic Name：人皮膚淋巴細胞相關抗原(CLA)ELISA試劑盒
This kit can be used for determination of serum, plasma and liquid samples Organization Content.
The experimental principle:
The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.
Materials provided with the kit
Materials provided with the kit    48determinations    96 determinations    Storage
User manual    1    1    
Closure plate membrane    2    2    
Sealed bags    1    1    
Microelisa stripplate    1    1    2-8℃
Standard：360ng/L    0.5ml×1 bottle    0.5ml×1 bottle    2-8℃
Standard diluent    1.5ml×1 bottle    1.5ml×1 bottle    2-8℃
HRP-Conjugate reagent    3ml×1 bottle    6ml×1 bottle    2-8℃
Sample diluent    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution A    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution B    3ml×1 bottle    6ml×1 bottle    2-8℃
Stop Solution    3ml×1 bottle    6ml×1 bottle    2-8℃
wash  solution    （20ml×20 fold）
×1bottle    （20ml×30 fold）
×1bottle    2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.人皮膚淋巴細胞相關抗原(CLA)ELISA試劑盒
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)
2.add sample：Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well. 
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Calculate：
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Storage and validity
1．Storage：  2-8℃.
2．validity： six months.

人皮膚淋巴細胞相關抗原(CLA)ELISA試劑盒其它產品SFRS7 Antibody    Q16629    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SFRS8 Antibody    Q12872    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SGK269 Antibody    Q9H792    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SH2D2A Antibody    Q9NP31    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SH3TC1 Antibody    Q8TE82    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SHANK2 Antibody    Q9UPX8    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SHB Antibody    Q15464    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SHD Antibody    Q96IW2    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SIRPG Antibody    Q9P1W8    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SirT1 Antibody    Q96EB6    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB IHC ELISA    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
SIRT2 Antibody    Q8IXJ6    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SIRT3 Antibody    Q9NTG7    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC10A7 Antibody    Q0GE19    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC15A1 Antibody    P46059    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC16A2 Antibody    P36021    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC17A2 Antibody    O00624    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC22A5 Antibody    O76082    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC24A1 Antibody    O60721    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC24A3 Antibody    Q9HC58    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC24A4 Antibody    Q8NFF2    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC24A6 Antibody    Q6J4K2    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC25A11 Antibody    Q02978    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC25A31 Antibody    Q9H0C2    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC25A6 Antibody    P12236    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC28A2 Antibody    O43868    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB IHC ELISA    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
SLC30A4 Antibody    O14863    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB IHC ELISA    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
SLC30A8 Antibody    Q8IWU4    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC30A9 Antibody    Q6PML9    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC30A9 Antibody    Q9Y6R2    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC38A2 Antibody    Q96QD8    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC39A1 Antibody    Q9NY26    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC39A7 Antibody    Q92504    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC39A9 Antibody    Q9NUM3    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC4A11 Antibody    Q8NBS3    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000
SLC4A8/10 Antibody    Q2Y0W8/Q6U841    美國AssayBiotech    25ug/50ug/100ug    1 mg/ml    WB ELISA    WB: 1:500~1:1000 ELISA: 1:10000